ABSTRACT
@#Upconversion nanoparticles(UCNPs)doped with rare earth elements have advantages in biose-nsing because of their good fluorescence stability,biocompatibility and avoidance of background fluorescence. Therefore,fluorescence resonance energy transfer(FRET)system based on upconversion particles(UCNPs based FRET)has been widely used in biological detection. This paper reviews the application and prospect of UCNPs based FRET in biological detection of biotoxins,hormones,proteins,nucleic acids,bacteria,and so on.
ABSTRACT
Rolling circle amplification is a rapid, sensitive and isothermal single-stranded DNA amplification technique that can be used with staining or probes to amplify the detection signal. This technology has been widely used in biological detection and other aspects. The present paper introduces how to design rolling circle amplification, summarize its application in the detection of pathogens, nucleic acid tumor markers, proteins, biological small biomolecules, and viruses in recent years and prospects for future development.
Subject(s)
DNA, Single-Stranded , Nucleic Acid Amplification TechniquesABSTRACT
Lateral flow chromatography is a new rapid detection and sensing technology,has the advantages of low cost,convenient to use and rapid detection.This method can better meet the requirements of quality and safety surveillance and on-site rapid screening by comparing with the physical and chemical detection methods which are expensive,complicated to operate and require high detection environment.In this paper,the lateral flow chromatography strips were introduced including the test methods,the classification of marking materials and their practical applications in various fields.The researches on the application of lateral flow chromatography strips in biological and food safety testing was reviewed including the advantages and the current research status.The existing bottlenecks and the future development direction were also analyzed.These can provide insight for the further application and in-depth research of strip rapid detection technologies.
ABSTRACT
The screening with quality control of solubilizing excipient is one of the reasons that restrict the development of Chinese materia medica (CMM) injection. In order to establish an screening and quality evaluation system for solubilizing excipient so as to ensure the safety and utility of CMM injection in clinic, the author introduced a new method which meets with the characteristics of CMM injection, for evaluating the quality of solubilizing excipient. This original method consists of the sequential analysis of "routine examination-physical property-chemical analysis-biological detection", laying particular emphasis on the biological detection. This research is expecting to provide ideas and technical reference for the safety evaluation of CMM injection.